(I).Education Section - Key Results

1.Planning for the improvement of biotechnology education in our university

Since 2006, the Center has been responsible for the planning and implementation of the biotechnology talent training and teaching improvement program promoted by the Executive Yuan, and has received annual subsidies from the Information and Technology Education Department, Ministry of Education (hereinafter referred to as the ITE Department). In accordance with the National Biotechnology Industry and Talent Cultivation Plan, which is a four-year program promoted by the government, the CBB has integrated resources from the College of Medicine, the College of Bioscience and Biotechnology, the College of Management, the College of Planning and Design, the Innovation Headquarters, and the talents and faculty of outstanding enterprises and entrepreneurs from outside the university. Every year, we have been successful in obtaining and implementing biotechnology talent training programs with excellent results.

2006: Implemented the Ministry of Education’s “Advanced Biotechnology Talent Training Program”.

2. Organizing biotechnology courses

Biotechnology is an emerging interdisciplinary research field with applications in agriculture, food and pharmaceutical industries, specialty chemicals, environmental industries, and energy industries. Since biotechnology industry has a profound impact on the future life, health, wellbeing, and environmental quality of human beings, it has been recognized as the most promising high-tech industry in the 21st century. The government of Taiwan recognizes the importance of the biotechnology industry to the future economic development, and promulgated the “Act for the Development of Biotech and Pharmaceutical Industry” in 1995 and 2007 respectively to effectively promote this industry. In order to meet the needs of promoting national key technologies and cultivating talents for future industrial development, the Center integrates resources from both management and biotechnology to cultivate interdisciplinary talents with both biological and management science to meet the needs of the industry.

(II).Planning Section - Key Results Report

1.Preparing for the Planning and Execution of the Relocation of the CBB (2018/04/01)

The CBB is located on the 9th and 10th floors of the Equipment Building on the campus of National Cheng Kung University Tzu-Chiang Campus. Planned since 2009, the Bioscience and Biotechnology Building, located on the Li-Hsing campus of National Cheng Kung University (NCKU) unites all the related departments and centers of the College of Bioscience and Biotechnology in a single block, thereby enhancing efficiency and generating greater benefits for teaching, industry-academia collaboration, research, and mutual collaboration support. It can increase efficiency significantly and generate greater benefits. The Bioscience and Biotechnology Building has been completed and the Center was officially relocated to the Building in July 2018; all administrative operations are now officially operating in the Building.

(III).Research Section - Key Results Report

1.Center for Microalgae Biotechnology and Engineering

1. Implementing international cooperation programs
   1. International cooperation project with Kobe University, Japan: “Isolation, characterization and optimal cultivation of indigenous carbohydrate-rich microalgae used as feedstock for bioethanol production” (2011.08.01-2015.07.31)
   2. Collaborated with Asia Nutrition Technologies Co., Ltd. on project “Isolation, characterization and optimal cultivation of piggery wastewater tolerant microalgae used as feedstock for wastewater treatment and feed production” (2016.11-2018.10). and signed an international technology transfer agreement “High piggery wastewater tolerance photo-heterotrophic microalgae” (2017.07-2019.06) for an amount of NT$420,000.
   3. Execution of cooperation project with Sophie’s Kitchen in Singapore (2019.01-present) and signed international technology transfer agreement “High protein content photo-heterotrophic microalgae” (2019.08-2029.07) for NT$2,000,000.
2. Signed LOI to cooperate with Taiwan’s heavyweight petrochemical industry players, such as Formosa Petrochemical Corp., Formosa Environmental Technology Corp., China Petrochemical Development Corporation, and Hong Jing Environment Company.
3. Implementation of the Ministry of Science and Technology’s Green Energy Program - Research and Development and Demonstration of Industrial Pilot Waste Energy Cogeneration Technology (1/2) (2021.07-2022.05).
4. Execution of the Ministry of Science and Technology’s National Energy Program

1. High Efficiency Waste Energy Conversion and Utilization System (2019.04-2020.12)
2. Carbon Dioxide High Value Utilization and Commercialization Technology Development by Microalgae Carbon Sequestration and Biorefinery Platform (2017.01-2018.12)
3. Integrated Carbon Dioxide Mineral Sequestration and Carbon Fixation by Algal Cultivation Using Bos to Create Sea Forest and Marine Farm (2016.01-2018.12)

5. The cumulative value of technology transfers exceeds NT$30 million.
6. Published nearly 100 international journals (more than 60% of international journals with ranking < 10%).
7. Execution of MOST programs

1. Development and High Value Utilization of Microalgae Fucoxanthin Mass Production System (2021.08-2024.07)
2. Development of a Consumer Production Framework Based on Recycling of Agricultural Organic Materials and a Green Circular Economy Model - Application of Biorefinery Technology to Convert Agricultural by-Products for Succinic Acid Production (2021.08-2024.07)
3. Application Development of Smart Aquaculture and Microalgae Circular Economy - Application Development of Smart Aquaculture and Microalgae Recycling Economy (2020.11-2021.10)
4. Implementation of the Ministry of Science and Technology’s Value Creation Program - High Performance Treatment of Industrial Wastewater with Innovative Microbial Technology (2019.11-2020.10)
5. Ministry of Science and Technology Outstanding Young Scholars Program - Development of Microalgae Protein Production System for Sustainable Innovation with Commercial Viability (2017.08-2020.07)
6. Development and Application of New Functional Aquatic Feed Additives (1/2) (2019.08-2020.07)
7. Development of Key Technologies for High-Value Microalgae Carbon Circular Materials (3/3) (2019.06-2020.05)
8. Sustainable Development of Aquaculture Blue Economy Through Green Engineering Technology of Carbon Sequestration by Microalgae Cultivation - Development of Innovative CO₂ Capture and Purification Technology and High Efficiency Microalgae Pre-Treatment for Active Ingredient Extraction and Dosage Form Preparation (2019.08-2020.07)
9. Development of a Circular Economic Model of Microalgae Biorefinery Technology for Livestock Wastewater (2018.08-2019.07)
10. Development of Key Technologies for High Value Microalgae Carbon Circular Materials (2/3) (2018.06-2019.05)
11. Development and Application of New Functional Aquatic Feed Additives--Development and Application of New Functional Aquatic Feed Additives (2/2) (2018.05-2019.04)
12. Development of Wastewater Microalgae Biorefining Technology with the Concept of Circular Economy: Technical Feasibility Assessment of Treating Piggery Wastewater With Photoheterotrophic Microalgae Culturing System (2017.08-2018.07)
13. Developing a Sustainable and Innovative Microalgae-Based Lutein Production System with Commercial Viability (2016.08-2017.07)
14. Development of Innovative Production Technology of Microalgae Biodiesel Using Agricultural Wastes as Biofuel (I-II) ( 2015.08-2017.07)
15. Development of a Cost-Effective and Innovative Microalgae Lutein Production System (2015.08-2016.07)
16. Development of a Low Cost and High CO2 Removal Efficiency Microalgae Phycocyanin Production System (2014.08-2015.07)

8. Signed an industry-academic cooperation project with Formosa Plastics Corporation on “Validation of PVC Wastewater Treatment Efficiency” (2021.09-2022.08)
9. Signed an industry-academia cooperation project with the Foundation of Taiwan Sugar Association for “Validation Program for Under-Solar Panel Bio-System Aquaculture Equipment” (2021.08-2022.04)
10. Signed an industry-academia cooperation project with Yongfeng Yukuang Co., Ltd. on “Building a Circular Economic Model Combining Solar Energy and Aquaculture” (2021.07-2022.12)
11. Signed an industrial-academic cooperation project with Elimbio Co., Ltd. on “Feasibility Assessment in the Integration of Solar Energy Generation and Aquaculture” (2021.04-2022.06)
12. Signed an industrial-academic cooperation project with Formosa Plastics Corporation “Study of Heavy Metals and Ammonia Nitrogen Treatment Efficiency in MMA Wastewater” (2021.02-2021.06).
13. Signed an industrial-academic cooperation project with Formosa Petrochemical Corporation, “Research Project of Microalgae Liquid Fertilizer for High Value Crop Planting” (2021.01-2021.10)
14. Signed an industry-academia cooperation project with Foundation of Taiwan Sugar Association, “Water-based Solar Circular Economy Model Project” (2019.08-2020.01)
15. Executed academic collaboration projection of the Council on Agriculture, titled “Microalgae Wastewater Treatment and Value-Added Reuse - Development of High Performance Microalgae Wastewater Treatment and Microalgae Conversion Platform” (2019.03-2019.12)
16. Signed an industrial-academic cooperation project with the Nanoplus Limited (Cayman) Taiwan Branch “Establishment of the Nano Microalgae Circular Economy Project” (2017.12-2019.06)
17. Execution of CPC Project “Biomass Material Production Technology and Product Development Research” (2017.06-2018.11)
18. Execution of the Metal Industries Research & Development Centre Cooperation Program

1. Hydrothermal Liquefied Oil Products Testing and Analysis (2017.04-2017.11)
2. Industrial Wet Biomass Waste Material Source Analysis (2018.04-2018.11)

19. Signed an industry-academia cooperation project with Greenyn Biotechnology Co., Ltd. on “Optimization of Microalgae Extracting and Processing Conditions for Outdoor Cultivation of High Nutritional Value Microalgae Species and Extraction of Algal Components” (2014.03.01-2015.12.31)
20. Executed the MOEA “Green Biomass Energy Technology R&D and Industry Promotion Project” (2013.07.01-2014.12.31)
21. Signed an industry-academia cooperation plan with the Industrial Technology Research Institute
    1. “Analysis of the Characteristics of Active Substances That Induce Biological Cell Destruction” (2014.06-2014.12)
    2. “Hydrothermal Liquefied Oil Products Testing and Analysis” (2017.04-2017.11)
       

2.Protein Crystallography Laboratory

1. In collaboration with the Protein Production and X-ray Structure Analysis Core Facility at the Institute of Biological Chemistry, Academia Sinica, the Life Sciences Group and Biocrystallography Facility at the Synchrotron Radiation Research Center in Hsinchu, and the SPring-8 Laboratory in Japan, the CBB is engaged in the analysis of protein crystals and crystal structures, exploring protein functions and mechanisms of action, and establishing a database of structural biological information for molecular modeling, drug screening, and biomedical research applications.
2. From 2011.08 to 2015.05, we started to invest NT$3-5 million dollars per year in the Protein Crystallography Laboratory of National Cheng Kung University through the National Biotechnology and Pharmaceutical Science and Technology Program, and further upgrade the laboratory to the scale of the national-level “Southern Protein Core Laboratory”. 
3. Collaborated with GE Medical Systems Taiwan to conduct “Purification Application Technology Seminar” and promote the “Protein Crystallography Lab”.
4. Constructing a platform for the production of heterologous genetically recombinant proteins from microalgae.
5. We are building a platform for the production of expensive medicinal natural substances by genetically modified microorganisms.
6. Industry-academia project on structural analysis of a novel therapeutic cervical cancer vaccine associated with mastovirus structural proteins in collaboration with HealthBanks Biotech Co., Ltd.
7. Functional and structural study on the regulation of staphylococcus aureus biofilm formation and pathogenic factors by SrrAB Regulatory System (MOST 2013.8-2016.7 Three-Year Project: NT$4.8 million)
8. Side-directed Mutagenesis, Engineering and Production of Carboxylesterase (industry-academia collaboration 2013.8-2014.4 NT$620,000) (Collaboration between Credo Biomedical Pte Ltd (Singapore) and NCKU Research and Development Foundation.)
9. Development of protein drugs for the treatment of lung cancer and polycystic kidney disease (Taiwan Protein Program - Strengthening Protein Research in Southern Taiwan 2016.1-2019.12, approximately NT$800,000-NT$1.2 million per year, funded by Academia Sinica).
10. “Development and Application of NGAL Recombinant Protein for the Treatment of Polycystic Kidney Disease”, collaboration between CBB, Dr. Yuan-Yow Chiu, National Cheng Kung University Hospital and Prof. Hsiu-Mei Hsieh, National Taiwan Normal University in MOST Industry-Academia Collaboration Project (2018.06-2020.05 2-year project, NT$2.55 million per year, collaboration with PharmaEssentia Corp.)
11. Ongoing patent applications for “Use of Neutrophil Gelatinase-Associated Lipocalin for Preparing Drug” has been granted in Taiwan, the U.S., Europe (Germany, England, France) and Japan; the Canadian patent is still under review.
12. Published 46 articles in international journals (21 articles from Dr. Wen-Yi Jeng and 25 articles from Dr. Chun-Jung Chen)